Bal Ra., Türk G.a, Tuzcu M.a, Yilmaz O.a, Ozerkan I.a, Kuloglu T.a, Gür S.a, Nedzvetsky V.b, Tykhomyrov A.c, Andrievsky G.c, Baydas G.d, Naziroglu M.e
Diabetes mellitus (DM) is considered to be a premature aging process and characterized by producing male sexual dysfunction. Searching for the agents that could alleviate diabetes-induced impact on reproductive system is yet the important area of inquiry. The present study was performed to evaluate whether antioxidant hydrated C60 fullerene (C60HyFn) alleviate testicular dysfunction induced by streptozotocyn (STZ)-diabetes in rats. Sexually mature Wistar male albino rats were taken for experiment and divided into four groups as follows (n = 6 per group): (1) control group (untreated animals), (2) C60HyFn-treated nondiabetic group, (3) STZ-diabetic group, and (4) C60HyFn-treated diabetic group. Diabetes in animals of 3-rd and 4-th groups was induced by a single intraperitoneal injection of a buffered solution of STZ at a dose of 50 mg/kg body weight. Once sugar levels achieved consistent value after STZ injection (more than 300 mg/dl), rats in the 2-nd and 4-th groups were treated with C60HyFn (in the form of drinking water) at the dose of 4 μg/kg daily for 5 weeks. In diabetic rats, levels of serum testosterone, testicular reduced glutathione (GSH) and alpha-tocopherol were significantly reduced and testicular lipid peroxidation level was increased (p < 0.001). Treatment of diabetic rats with C60HyFn resulted in significant corrective effects on these parameters towards the control levels. Furthermore, C60HyFn treatment in diabetic and nondiabetic rats resulted in considerable elevations of some important polyunsaturated fatty acids. However, administration of C60HyFn to diabetic animals did not make any significant change in blood glucose level. In diabetic rats, relative weights of right cauda epididymis, seminal vesicles, prostate, sperm motility and epididymal sperm concentration were significantly less than those of control group, but which were restored in the fourth group treated with C60HyFn (p < 0.001). Marked histopathological changes including degeneration, desquamation, disorganisation and reduction in germinal cells, interstitial oedema and congestion were evident in the testis of diabetic rats, but C60HyFn treatment resulted in recovery of histopathological changes. In conclusion, we have presented for the first time substantial evidence that administration of C60HyFn significantly reduces diabetes-induced oxidative stress and associated complications such as testicular dysfunction and spermatogenic disruption.