Roshina N. V., Symonenko A. V., Pasyukova E. G.
Institute of Molecular Genetics of RAS, Moscow, Russia;
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Earlier, we demonstrated that several genes participating in regulation of the nervous system development are involved in lifespan control. An insertion of the P{GT1} vector 300 bp downstream of the structural part of escargot (esg) that encodes an RNA polymerase II transcription factor and insertions of P-based vectors in the structural part of aPRC that encodes atypical protein kinase C were associated with increase in male and female lifespan. Both esg and aPKC are involved in regulation of a crucial step in the nervous system development, asymmetric neuroblast division (AND). We suggested that other genes interacting with esg and aPKC during AND could be important for lifespan control. inscrutable (insc) is a gene essential for AND. We demonstrated that an insertion of the P{EPgy2} vector in the structural part of insc prolonged female lifespan. Analysis of lifespan of heterozygous esg and insc double mutants allowed us to predict that these genes interact in the course of lifespan determination. Decrease of esg transcription was shown to be associated with lifespan increase in mutant esg females and males. Mutant insc females with increased lifespan also demonstrated decrease in esg transcript amount. This result confirmed both interaction between esg and insc and association of prolonged lifespan with low esg expression. aPKC is directly phosphorylated by GSK3β (glycogen synthase kinase 3) encoded by shaggy (sgg), which further provides AND. We demonstrated that several insertions of P-based vectors in the structural part of sgg were associated with alterations of male and female lifespan. Altogether, our recent findings indicate that genes affecting AND during early steps of development could influence longevity of Drosophila adults.